Read e-book online Laboratory Methods in Enzymology: Protein Part A PDF

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By Jon Lorsch

ISBN-10: 0076687953

ISBN-13: 9780076687954

ISBN-10: 0124200702

ISBN-13: 9780124200708

The significantly acclaimed laboratory general for nearly 50 years, Methods in Enzymology is without doubt one of the such a lot hugely revered guides within the box of biochemistry. each one quantity is eagerly awaited, usually consulted, and praised via researchers and reviewers alike. Now with over 520 volumes and 40,000 chapters within the assortment, a lot of the cloth remains to be appropriate this present day and is really an important booklet for researchers in all fields of lifestyles sciences, together with microbiology, biochemistry, melanoma examine, and genetics, simply to identify a few.

In this quantity, quantity 545, we've introduced jointly a few center protocols targeting protein, conscientiously written and edited by way of experts.

  • Indispensable device for the researcher
  • Carefully written and edited by means of specialists to include step by step protocols
  • Brings jointly a few center protocols targeting protein

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Read or Download Laboratory Methods in Enzymology: Protein Part A PDF

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Extra info for Laboratory Methods in Enzymology: Protein Part A

Example text

5 h in a SW40 rotor. 5-ml microcentrifuge tubes. 5-ml aliquot from each fraction to a separate Microcon YM-3 centrifugal concentrator. Flash-freeze the remainder of each fraction in liquid nitrogen and store at À80  C. 6 Concentrate the fractions until a minimal volume remains (about 20 ml). Add 1Â SDS sample buffer to 100 ml. 3. Tip Use a stock solution of 50% sucrose (w/v) to prepare the gradient steps. See Fig. 3 for the flowchart of Step A2. 2 Flowchart of Step A1. 3 Flowchart of Step A2. 7.

2007). A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes. Proteomics, 7, 4228–4234. , et al. (2010). Quantitative proteomic survey of endoplasmic reticulum in mouse liver. Journal of Proteome Research, 9, 1195–1202. Taylor, S. , et al. (2003). Characterization of the human heart mitochondrial proteome. Nature Biotechnology, 21, 281–286. Taylor, S. , Warnock, D. , Glenn, G. , et al. (2002). An alternative strategy to determine the mitochondrial proteome using sucrose gradient fractionation and 1D PAGE on highly purified human heart mitochondria.

7 Tip 6. 4 Tip 7. 1 Tip References Source References 66 67 67 68 70 70 70 71 71 71 72 72 72 72 72 72 72 73 74 74 74 75 75 75 Abstract In order to measure protein half-life by pulse-chase analysis, cells are incubated with labeled precursors, [35 S]-methionine and [35 S]-cysteine, for a short time (pulse), after which they are washed from the labeled compound and incubated with excess cold precursor (chase). The decay of the amount of protein labeled during the pulse period is measured during the chase with cold precursor.

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Laboratory Methods in Enzymology: Protein Part A by Jon Lorsch

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